This works provides an expansion in the area of aliphatic polyimides with the objective to be used in
semidry lithium ion polymer batteries [9].
Then the samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred on nitrocellulose membranes (BIO-RAD Membrane, Hercules, CA, USA; 0.22 [micro]m) by
semidry transfer cell (BIO-RAD, USA).
Then, equal concentrations of protein samples were separated by electrophoresis on sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) with a
semidry transfer system (Bio-Rad Laboratories, USA).
It has
semidry and subhumid climates, with an annual rainfall of 850 mm, minimum temperature of 16 C and maximum of 32 C, and has a vegetation composed mostly of tropical deciduous and subdeciduous forests.
A
semidry transfer unit (Hoefer Scientific, Holliston, MA) was used to transfer the separated proteins onto a PVDF membrane (Bio-Rad) at 15 V for 50 mins.
Proteins were then transferred from gel to PVDF membrane (Immobilon P; Millipore, Bedford, MA) in a
semidry electroblotting system (Bio-Rad) at 25 V for 50 min.
The separated proteins were transferred to PVDF membranes by using a Bio-Rad
semidry transfer unit (Bio-Rad, Hercules, CA, USA).
The proteins were transferred onto the membrane by the
semidry method.
At present, there were mainly dry, wet, and
semidry methods for the preparation of starch phosphate at home and abroad.